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igmscd1ser100ml serum  (Innovative Research Inc)


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    Innovative Research Inc igmscd1ser100ml serum
    Igmscd1ser100ml Serum, supplied by Innovative Research Inc, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/igmscd1ser100ml+serum/Innovative+Grade+US+Origin+Mouse+CD1+Serum/pmc12419644__pone%2E0331445%2Es001-19-193-191
    Average 93 stars, based on 12 article reviews
    igmscd1ser100ml serum - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Saline:

    Article Title: Evaluating antibody mediated opsonophagocytosis of bacteria via lab protocol: RAW 264.7 cell phagocytosis assay
    Article Snippet: .. Our lab has also used phagocytosis assays to evaluate immunostimulatory compounds, and in such cases our negative controls are cells that are not given IFN γ, and positive controls are given IFN γ, with experimental groups given our test compounds instead. protocols.io | https://dx.doi.org/10.17504/protocols.io.yxmvmmr16v3p/v1 August 20, 2025 2/10 Materials Materials and Media: Item Vendor Catalog number Description RAW 264.7 cells ATCC TIB 71 Phagocyte cell line used for invitro phagocytosis assays DMEM cell media Thermofisher 11965126 Supplement with 10% FBS toprepare complete media Murine IFN γ Peprotech 315 05 Used to activate RAW 264.7cells Phosphate Buffered Saline PBS VWR 45000 436 General purpose saline buffer Hanks Buffered Salt Solution HBSS VWR 45001 101 Supplemented buffer for washing and maintaining cells Circular glass coverslips VWR 48380 046 18 mm circular coverslips thatfit into a 12 well plate Vectamount Vector laboratories H 5501 60 A wet mount solution that mounts coverslips to microscope slides HEMA I VWR 10143 236 A stain used for visualizingphagocytosis HEMA I I VWR 10143 238 A stain used for visualizingphagocytosis Triton-X 100 VWR AAA16046 AE Dilute to 0.1% in PBS to make alysis buffer CD 1 Mouse serum InnovativeResearch, Inc. IGMSCD1SER100ML Serum for supporting cells during the phagocytosis process Light microscope and mounted camera Leica ICC50 HD For the microscopy-based assay, a high-quality mounted camera is recommended RAW 264.7 Cells ATCC Catalog #TIB 71 DMEM, high glucose Thermo Fisher Catalog #11965126 Mouse IgG Isotype Control, FITC peprotech Catalog #31505 Dulbeccos phosphate-buffered saline DPBS VWR International Avantor) Catalog #45000 436 HBSS Hankʼs Balanced Salt Solution) 1X with Calcium and Magnesium, without Phenol red VWR International Avantor) Catalog #45001 101 Cover glasses, round VWR International Avantor) Catalog #48380 046 VectaMount® AQ Aqueous Mounting Medium Vector Laboratories Catalog #H 5501 60 Wright-Giemsa stain, VWR® Quick IIITM Solution I for hematology VWR International Avantor) Catalog #10143 236 A B C D protocols.io | https://dx.doi.org/10.17504/protocols.io.yxmvmmr16v3p/v1 August 20, 2025 3/10 Wright-Giemsa stain, VWR® Quick IIITM Solution II for hematology VWR International Avantor) Catalog #10143 238 TRITONTM X 100 Polyethylene glycol tert-octylphenyl ether) VWR International Avantor) Catalog #AAA16046 AE protocols.io | https://dx.doi.org/10.17504/protocols.io.yxmvmmr16v3p/v1 August 20, 2025 4/10 ..

    Microscopy:

    Article Title: Evaluating antibody mediated opsonophagocytosis of bacteria via lab protocol: RAW 264.7 cell phagocytosis assay
    Article Snippet: .. Our lab has also used phagocytosis assays to evaluate immunostimulatory compounds, and in such cases our negative controls are cells that are not given IFN γ, and positive controls are given IFN γ, with experimental groups given our test compounds instead. protocols.io | https://dx.doi.org/10.17504/protocols.io.yxmvmmr16v3p/v1 August 20, 2025 2/10 Materials Materials and Media: Item Vendor Catalog number Description RAW 264.7 cells ATCC TIB 71 Phagocyte cell line used for invitro phagocytosis assays DMEM cell media Thermofisher 11965126 Supplement with 10% FBS toprepare complete media Murine IFN γ Peprotech 315 05 Used to activate RAW 264.7cells Phosphate Buffered Saline PBS VWR 45000 436 General purpose saline buffer Hanks Buffered Salt Solution HBSS VWR 45001 101 Supplemented buffer for washing and maintaining cells Circular glass coverslips VWR 48380 046 18 mm circular coverslips thatfit into a 12 well plate Vectamount Vector laboratories H 5501 60 A wet mount solution that mounts coverslips to microscope slides HEMA I VWR 10143 236 A stain used for visualizingphagocytosis HEMA I I VWR 10143 238 A stain used for visualizingphagocytosis Triton-X 100 VWR AAA16046 AE Dilute to 0.1% in PBS to make alysis buffer CD 1 Mouse serum InnovativeResearch, Inc. IGMSCD1SER100ML Serum for supporting cells during the phagocytosis process Light microscope and mounted camera Leica ICC50 HD For the microscopy-based assay, a high-quality mounted camera is recommended RAW 264.7 Cells ATCC Catalog #TIB 71 DMEM, high glucose Thermo Fisher Catalog #11965126 Mouse IgG Isotype Control, FITC peprotech Catalog #31505 Dulbeccos phosphate-buffered saline DPBS VWR International Avantor) Catalog #45000 436 HBSS Hankʼs Balanced Salt Solution) 1X with Calcium and Magnesium, without Phenol red VWR International Avantor) Catalog #45001 101 Cover glasses, round VWR International Avantor) Catalog #48380 046 VectaMount® AQ Aqueous Mounting Medium Vector Laboratories Catalog #H 5501 60 Wright-Giemsa stain, VWR® Quick IIITM Solution I for hematology VWR International Avantor) Catalog #10143 236 A B C D protocols.io | https://dx.doi.org/10.17504/protocols.io.yxmvmmr16v3p/v1 August 20, 2025 3/10 Wright-Giemsa stain, VWR® Quick IIITM Solution II for hematology VWR International Avantor) Catalog #10143 238 TRITONTM X 100 Polyethylene glycol tert-octylphenyl ether) VWR International Avantor) Catalog #AAA16046 AE protocols.io | https://dx.doi.org/10.17504/protocols.io.yxmvmmr16v3p/v1 August 20, 2025 4/10 ..

    Staining:

    Article Title: Evaluating antibody mediated opsonophagocytosis of bacteria via lab protocol: RAW 264.7 cell phagocytosis assay
    Article Snippet: .. Our lab has also used phagocytosis assays to evaluate immunostimulatory compounds, and in such cases our negative controls are cells that are not given IFN γ, and positive controls are given IFN γ, with experimental groups given our test compounds instead. protocols.io | https://dx.doi.org/10.17504/protocols.io.yxmvmmr16v3p/v1 August 20, 2025 2/10 Materials Materials and Media: Item Vendor Catalog number Description RAW 264.7 cells ATCC TIB 71 Phagocyte cell line used for invitro phagocytosis assays DMEM cell media Thermofisher 11965126 Supplement with 10% FBS toprepare complete media Murine IFN γ Peprotech 315 05 Used to activate RAW 264.7cells Phosphate Buffered Saline PBS VWR 45000 436 General purpose saline buffer Hanks Buffered Salt Solution HBSS VWR 45001 101 Supplemented buffer for washing and maintaining cells Circular glass coverslips VWR 48380 046 18 mm circular coverslips thatfit into a 12 well plate Vectamount Vector laboratories H 5501 60 A wet mount solution that mounts coverslips to microscope slides HEMA I VWR 10143 236 A stain used for visualizingphagocytosis HEMA I I VWR 10143 238 A stain used for visualizingphagocytosis Triton-X 100 VWR AAA16046 AE Dilute to 0.1% in PBS to make alysis buffer CD 1 Mouse serum InnovativeResearch, Inc. IGMSCD1SER100ML Serum for supporting cells during the phagocytosis process Light microscope and mounted camera Leica ICC50 HD For the microscopy-based assay, a high-quality mounted camera is recommended RAW 264.7 Cells ATCC Catalog #TIB 71 DMEM, high glucose Thermo Fisher Catalog #11965126 Mouse IgG Isotype Control, FITC peprotech Catalog #31505 Dulbeccos phosphate-buffered saline DPBS VWR International Avantor) Catalog #45000 436 HBSS Hankʼs Balanced Salt Solution) 1X with Calcium and Magnesium, without Phenol red VWR International Avantor) Catalog #45001 101 Cover glasses, round VWR International Avantor) Catalog #48380 046 VectaMount® AQ Aqueous Mounting Medium Vector Laboratories Catalog #H 5501 60 Wright-Giemsa stain, VWR® Quick IIITM Solution I for hematology VWR International Avantor) Catalog #10143 236 A B C D protocols.io | https://dx.doi.org/10.17504/protocols.io.yxmvmmr16v3p/v1 August 20, 2025 3/10 Wright-Giemsa stain, VWR® Quick IIITM Solution II for hematology VWR International Avantor) Catalog #10143 238 TRITONTM X 100 Polyethylene glycol tert-octylphenyl ether) VWR International Avantor) Catalog #AAA16046 AE protocols.io | https://dx.doi.org/10.17504/protocols.io.yxmvmmr16v3p/v1 August 20, 2025 4/10 ..

    Light Microscopy:

    Article Title: Evaluating antibody mediated opsonophagocytosis of bacteria via lab protocol: RAW 264.7 cell phagocytosis assay
    Article Snippet: .. Our lab has also used phagocytosis assays to evaluate immunostimulatory compounds, and in such cases our negative controls are cells that are not given IFN γ, and positive controls are given IFN γ, with experimental groups given our test compounds instead. protocols.io | https://dx.doi.org/10.17504/protocols.io.yxmvmmr16v3p/v1 August 20, 2025 2/10 Materials Materials and Media: Item Vendor Catalog number Description RAW 264.7 cells ATCC TIB 71 Phagocyte cell line used for invitro phagocytosis assays DMEM cell media Thermofisher 11965126 Supplement with 10% FBS toprepare complete media Murine IFN γ Peprotech 315 05 Used to activate RAW 264.7cells Phosphate Buffered Saline PBS VWR 45000 436 General purpose saline buffer Hanks Buffered Salt Solution HBSS VWR 45001 101 Supplemented buffer for washing and maintaining cells Circular glass coverslips VWR 48380 046 18 mm circular coverslips thatfit into a 12 well plate Vectamount Vector laboratories H 5501 60 A wet mount solution that mounts coverslips to microscope slides HEMA I VWR 10143 236 A stain used for visualizingphagocytosis HEMA I I VWR 10143 238 A stain used for visualizingphagocytosis Triton-X 100 VWR AAA16046 AE Dilute to 0.1% in PBS to make alysis buffer CD 1 Mouse serum InnovativeResearch, Inc. IGMSCD1SER100ML Serum for supporting cells during the phagocytosis process Light microscope and mounted camera Leica ICC50 HD For the microscopy-based assay, a high-quality mounted camera is recommended RAW 264.7 Cells ATCC Catalog #TIB 71 DMEM, high glucose Thermo Fisher Catalog #11965126 Mouse IgG Isotype Control, FITC peprotech Catalog #31505 Dulbeccos phosphate-buffered saline DPBS VWR International Avantor) Catalog #45000 436 HBSS Hankʼs Balanced Salt Solution) 1X with Calcium and Magnesium, without Phenol red VWR International Avantor) Catalog #45001 101 Cover glasses, round VWR International Avantor) Catalog #48380 046 VectaMount® AQ Aqueous Mounting Medium Vector Laboratories Catalog #H 5501 60 Wright-Giemsa stain, VWR® Quick IIITM Solution I for hematology VWR International Avantor) Catalog #10143 236 A B C D protocols.io | https://dx.doi.org/10.17504/protocols.io.yxmvmmr16v3p/v1 August 20, 2025 3/10 Wright-Giemsa stain, VWR® Quick IIITM Solution II for hematology VWR International Avantor) Catalog #10143 238 TRITONTM X 100 Polyethylene glycol tert-octylphenyl ether) VWR International Avantor) Catalog #AAA16046 AE protocols.io | https://dx.doi.org/10.17504/protocols.io.yxmvmmr16v3p/v1 August 20, 2025 4/10 ..

    Control:

    Article Title: Evaluating antibody mediated opsonophagocytosis of bacteria via lab protocol: RAW 264.7 cell phagocytosis assay
    Article Snippet: .. Our lab has also used phagocytosis assays to evaluate immunostimulatory compounds, and in such cases our negative controls are cells that are not given IFN γ, and positive controls are given IFN γ, with experimental groups given our test compounds instead. protocols.io | https://dx.doi.org/10.17504/protocols.io.yxmvmmr16v3p/v1 August 20, 2025 2/10 Materials Materials and Media: Item Vendor Catalog number Description RAW 264.7 cells ATCC TIB 71 Phagocyte cell line used for invitro phagocytosis assays DMEM cell media Thermofisher 11965126 Supplement with 10% FBS toprepare complete media Murine IFN γ Peprotech 315 05 Used to activate RAW 264.7cells Phosphate Buffered Saline PBS VWR 45000 436 General purpose saline buffer Hanks Buffered Salt Solution HBSS VWR 45001 101 Supplemented buffer for washing and maintaining cells Circular glass coverslips VWR 48380 046 18 mm circular coverslips thatfit into a 12 well plate Vectamount Vector laboratories H 5501 60 A wet mount solution that mounts coverslips to microscope slides HEMA I VWR 10143 236 A stain used for visualizingphagocytosis HEMA I I VWR 10143 238 A stain used for visualizingphagocytosis Triton-X 100 VWR AAA16046 AE Dilute to 0.1% in PBS to make alysis buffer CD 1 Mouse serum InnovativeResearch, Inc. IGMSCD1SER100ML Serum for supporting cells during the phagocytosis process Light microscope and mounted camera Leica ICC50 HD For the microscopy-based assay, a high-quality mounted camera is recommended RAW 264.7 Cells ATCC Catalog #TIB 71 DMEM, high glucose Thermo Fisher Catalog #11965126 Mouse IgG Isotype Control, FITC peprotech Catalog #31505 Dulbeccos phosphate-buffered saline DPBS VWR International Avantor) Catalog #45000 436 HBSS Hankʼs Balanced Salt Solution) 1X with Calcium and Magnesium, without Phenol red VWR International Avantor) Catalog #45001 101 Cover glasses, round VWR International Avantor) Catalog #48380 046 VectaMount® AQ Aqueous Mounting Medium Vector Laboratories Catalog #H 5501 60 Wright-Giemsa stain, VWR® Quick IIITM Solution I for hematology VWR International Avantor) Catalog #10143 236 A B C D protocols.io | https://dx.doi.org/10.17504/protocols.io.yxmvmmr16v3p/v1 August 20, 2025 3/10 Wright-Giemsa stain, VWR® Quick IIITM Solution II for hematology VWR International Avantor) Catalog #10143 238 TRITONTM X 100 Polyethylene glycol tert-octylphenyl ether) VWR International Avantor) Catalog #AAA16046 AE protocols.io | https://dx.doi.org/10.17504/protocols.io.yxmvmmr16v3p/v1 August 20, 2025 4/10 ..



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